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Lumbar puncture is an essential neurological investigation. The anatomy of the spinal canal allows cerebrospinal fluid (CSF) to be sampled from the lumbar region. We describe how, with adequate preparation and correct positioning of the patient, the procedure can be performed quickly and safely. The range of investigations that can be performed on the CSF and their interpretation will be discussed.Lumbar puncture is generally performed to obtain pressure measurements and to withdraw CSF in order to secure a sample of the fluid for cellular, chemical, and bacteriologic examination. In some cases lumbar puncture is used to administer spinal anesthetics or antibiotics or to inject air or a radiopaque or water-soluble contrast medium substance for myelography. Lumbar puncture plays an important role in the diagnosis of certain conditions, including hydrocephalus, meningitis, and subarachnoid hemorrhage
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