In order to study metabolic pathways and utilize them for biotechnology, it is frequently necessary to clone gene clusters that can be tens of kilobases in size. Traditional library construction, followed by screening and sub-cloning, is time consuming and costly. PCR amplification and assembly is more precise, quicker and cheaper, but the DNA polymerases used have an inherent error rate and incorrect fragment pairing can occur. Total gene synthesis can be rapid, but again sequence verification is necessary.
Gittins JR (2015) Recombineering Recovery – Large DNA Cloning by Closing the Circle In Vivo. Clon Transgen 4:139. doi:10.4172/2168-9849.1000139