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The current study was intended to assess the alteration in a range of physio-biochemical parameters happening all through the acclimatization procedure of in vitro raised Cardiospermum halicacabum plantlets. The microshoots were initially grown in Murashige and Skoog’s medium amended with Thidiazuron (0.3 μM) and rooted in 1/3 MS medium amplified with 0.5 μM Indole- 3-acetic acid (IAA). The plantlets thus obtained were successfully hardened and transferred to greenhouse. Alterations in the activities of antioxidant enzymes were also observed during acclimatization. Increase in Superoxide dismutase activity was observed in plantlets reaching its maximum at 7th day of acclimation. An incessant augmentation in the activity of Catalase, Ascorbate Peroxidase and Glutathione Reductase were also detected all the way through the period of adaptation. The current effort put forward a view that tissue-cultured plants of C. halicacabum build up an antioxidant enzymatic organization which determines there aptitude to survive in oxidative stress and an up regulation of these enzymes would help to reduce the built up of reactive oxygen species (ROS).
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