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Red cells from each donor sample was prepared as 5.0% suspension in low ionic strength saline (LISS) and Rh antigen (C, E, c, e) typing was performed by gel card (Diamed AG, Cressier sur Morat, Switzerland). Ten micro litre of donor cell suspension was added to micro tubes followed by centrifuge at 910 rpm for 10 minutes. Agglutinated cells forming a red line on the surface of gel or dispersed in gel are considered positive. A compact button of cells on bottom of the micro tube indicated the absence of the corresponding antigen.
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