Human have the microorganisms in the skin, gut and mouth. Human skin microbiome based on the 16S rRNA encoding gene can reveal bacterial species diversity. Also bacterial species in the skin have diverse and unique composition between individuals. We thought that a bacterial fingerprint obtained from surfaces including computer keyboards aids forensic individual identification in case of evidence deficiency. Next generation sequencing was used to analyze the bacterial community on objects and fingertips to match the object to the individual.