Natural dyes are obtained from dye plants and dye animals in nature. They were the primary colour source of textiles until the mid - to late 19^th century]. For a long time, natural dyes have been used for purposes such as the colouring of wool, mohair, cotton and silk fibres as well as fur and leather [. Anthraquinones, naturally occurring in the madder roots (Rubia tinctorum L.), have been used for dyeing fabrics or fibres especially to the colour red and they have also been used as a lake pigment rarely since ancient times .In addition to their antioxidant, antimicrobial, antifungal and hypotensive effects; anthraquinones have also been known for their various effects such as anticancer and skin diseases . Madder (Rubia tinctorum L.) and other alizarin-containing plants belong to the most important group of red dyestuffs found in nature all over the world. Many of these belong to the large plant family of the Rubiaceae [. The dye plant is mentioned in early literature on dyeing and textiles dyed with a madder type, dated to 3000 BC, have been found in Mohenjo-Daro in the Indus valley. Madder type dyestuffs belong to the group of mordant dyes which need a pre-treatment with a metal salt. The metal salts most in use are alum compounds

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