Rhamnolipids (RLs) are glycolipid biosurfactants produced by various bacterial species including some Pseudomonas sp. and Burkholderia sp. . The RLs are amphiphilic molecules highly diverse and those produced by Pseudomonas aeruginosa have been extensively studied. Because of their excellent surface activity and hydrocarbonsolubilizing properties, the physicochemical properties of RLs have received considerable interest.