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Volume 5, Issue 5 (Suppl) Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5th International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Rapid identification of natural hypouricemic compounds fromalfalfa extract using UPLC-MS/MS coupled
with molecular docking
Su-Jung Hsu1, Shu-Mei Lin1 and Ching-Kuo Lee2
1National Chiayi University, Taiwan
2Taipei Medical University, Taiwan
Xanthine Oxidase (XO) is a key enzyme that catalyzes the oxidation of xanthine and hypoxanthine to the end product (uric acid)
in purine metabolism and is the major target enzyme for the hyperuricemia treatment and gout arthritis prevention. For the
past five decades, allopurinol is the only available XO inhibitor for hyperuricemia treatment. It is of great interest to search for other
natural XO inhibitors. Recent pharmacological studies demonstrated that alfalfa extract exhibits a variety of bioactivities, including
neuroprotective, hypocholesterolemic, antioxidant, antiulcer, antimicrobial, hypolipidemic and estrogenic activity. It has been shown
to be effective for treating atherosclerosis, heart disease, stroke, cancer, diabetes and menopausal symptoms. The aims of our study
were to investigate the XO inhibiting potential and to identify the corresponding active components in alfalfa extract. The alfalfa
extract was first combined with xanthine oxidase before applying to UPLC-ESI-QTOF-MS/MS for fingerprint analysis and structure
identification of active compounds. The potential XO inhibitors from alfalfa extract were identified, including tricin and chrysoeriol.
The XO inhibition and antioxidant activities of these compounds were further predicted using molecular docking software. The
results revealed that tricin showed the lowest inhibition constant value (Ki) which means that this compound was predicted as the
strongest inhibitor of XO. The method established in this study, LC-MS technique combined with Molecular Docking, might be also
applied to rapid identification of anti-oxidative compounds and enzyme inhibiting agents from other natural resources in addition
to alfalfa.
Biography
Su-Jung Hsu is currently a PhD student at the Department of Food Science, National Chiayi University, Taiwan. Her Doctoral research is focused on nutraceutical
properties of alfalfa, including bioactive components purification, structure identification and bio-function evaluation. She acquires expertise in purification and
chemical structure identification of natural products. She has purified and identified numbers of biologically functional components, especially compounds
possessing tyrosinase and xanthine oxidase inhibition activity, from a variety of natural resources. She also conducted studies to investigate bioavailability of
the active compounds from alfalfa extract. Her recent research interest is to study metabolomics of alfalfa extract, using in vitro cell co-culture model comprising
enterocytes and hepatocytes in tandem with UPLC-ESI-QTOF-MS/MS technology.
Su-Jung Hsu et al., Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017