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conferenceseries
.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Up-regulation of miRNA-29a is critical for dihydromyricetin-mediated suppression of matrix
metalloproteinase-2 and metastatic ability in human oral cancer
Chiao-Wen Lin and Shun-Fa Yang
Chung Shan Medical University, Taiwan
O
ral cancer is the most prevalent cancer for middle-aged men in Taiwan and its delayed diagnosis has been shown to be associated
with poor survival rates. Therefore, developing a novel natural drug or drug therapy is vital. Dihydromyricetin (DHM), also
called ampelopsin is the most abundant flavonoid in
Ampelopsis grossedentata
. However, the anticancer effects and related molecular
mechanism of DHM in human oral cancer cells have not been reported. In this study, we investigated the effect of DHM on SCC-9
and SAS oral cancer cells and examined the potential inhibitory mechanisms involved. The results showed that DHM significantly
inhibited cell migration and invasion in two oral cancer cell lines. In addition, real-time PCR and western blot analyses suggested
that DHM inhibited MMP-2 mRNA and protein expression. Luciferase assay showed that MMP-2 promoter activity was inhibited
after DHM treatment. Moreover, a microRNA (miRNA) analysis showed that miRNA-29a was predominantly up-regulated after
DHM treatment. Inhibition of miRNA-29a significantly relieved MMP-2 and motility suppression was imposed by DHM treatment.
Furthermore, ectopic miRNA-29a expression in highly invasive cells decreased MMP-2 expression and invasive abilities. Taken
together, our results provide new insights into the role of DHM-induced molecular and epigenetic regulation in suppressing oral
cancer metastasis.
Biography
Chiao-Wen Lin is an Associate Professor of Institute of Oral Sciences, Chung Shan Medical University, Taiwan. She has received her PhD degree in Molecular
Biology. In particular, her researches have been focused on pharmacology, cancer metastasis, apoptosis and autophagy in oral cancer. She has published more
than 40 papers in reputed journals.
cwlin@csmu.edu.twChiao-Wen Lin et al., Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017