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Volume 5, Issue 5 (Suppl) Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5th International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Osteogenic activity of constituents from Taiwan native plant
Yi-Tzu Lin, Yi-Wen Mao and Mei-Hsien Lee
Taipei Medical University, Taiwan
Osteoblasts and osteoclasts are two main cells of bone remodeling. While osteoblasts play an important role in bone formation via
different signaling pathways, osteoclasts are responsible for bone resorption. Osteogenesis is characterized by the presence of a
number of markers like: Alkaline Phosphatase (ALP) and type-I collagen at the differentiation stage of osteoblasts, while osteopontin
and osteocalcin are actively expressed during mineralization (the mature stage). Uraria crinita (L.) Desv. ex DC. (Fabaceae) has been
used for long as an herbal medicine to treat bone dysplasia in children in Taiwan and China. In the present study, we investigated
the active constituents of the root from U. crinita by bio-guided isolation in primary human osteoblast (HOb) cells. Cell viability
was determined using the WST-8 assay. Osteogenic activity was evaluated in HOb cells using ALP assay and Alizarin red S staining
for mineralization. Gene expression was analyzed using real-time PCR. The results showed that 50% ethanolic extract of U. crinita
roots increased ALP and mineralization activities. Six compounds were purified by chromatography and identified to be as: one
phenolic acid, two flavone glycosides and three isoflavones from the active ethyl acetate fraction. Compound 4 (isoflavone) exhibited
significantly increasing ALP and mineralization activities in HOb cells and it also up-regulated the osteogenesis-related gene
expression. It may be considered to be the potential target for enhancing osteogenic activity in the future.
Biography
Yi-Tzu Lin has completed her Master’s degree in 2013 and currently pursuing PhD at Taipei Medical University, Taiwan. Her major research is isolation, identification
and purification of the active compounds and investigation of their bioactivities as well as the related mechanisms.
Yi-Tzu Lin et al., Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017