Methodology applied for product recovery depends upon when and where the product is formed, the purity of the product, use of the product, impurities that are present within the broth, price of the product and so on. âWhenâ is applicable for, with which phase of the cell cycle the product is formed and âwhereâ is applicable for, whether the product is intra-cellular or extra-cellular in its formation. Product recovery cost mainly guides the cost of the product in the market. More investment towards product recovery increases the cost of the product in the market. Even purity of the product and intended use of the product will increase the investment towards product recovery and purification. Various techniques involved in product recovery are filtration, centrifugation, precipitation, dialysis, solvent extraction, chromatographic and electrophoretic techniques, crystallization techniques etc. Products which is extra-cellular, need filtration of spent broth directly and if the product is intra-cellular, then the cells need to be lysed either by sonication or by centrifugation methods and then the resultant product is subjected to filtration and other purification techniques. As we get our resultant filtrate, then this filtrate is subjected for the rest of the purification process. Sometimes the investment we put towards purification also depends on, whether the product needs to be partially purified or completely purified. In this way, the desired product after its purification techniques done will be in its finalised form which is to be packed accordingly and then need to be marketed.
Open access to the scientific literature means the removal of barriers (including price barriers) from accessing scholarly work.
Open Access raises practical and policy questions for scholars, publishers, funders, and policymakers alike, including what the return on investment is when paying an article processing fee to publish in an Open Access articles, or whether investments into institutional repositories should be made and whether self-archiving should be made mandatory, as contemplated by some funders.
Last date updated on October, 2014