AN IMPROVED PROTOCOL FOR IN VITRO REGENERATION OF RUBIA CORDIFOLIA L VIA ORGANOGENESIS
|Suresh Khadke1, Shweta Rani2, Vithal Awad1, Neelambika Meti2, Elangbam Singh2, Aniket Kuvalekar1 and Abhay Harsulkar1*
|Corresponding Author: Abhay Harsulkar, E-mail: [email protected]|
|Received: 22 July 2013 Accepted: 31 August 2013|
An efficient protocol for the regeneration of Rubia cordifolia L via indirect organogenesis was developed. The traditional harvesting procedure of plant is destructive and needs urgent conservation. Here we report, an efficient protocol for regeneration and multiplication of plantlets, through indirect organogenesis in R. cordifolia L. Organogenic callus and adventitious shoots were raised on filter paper bridge in MS liquid medium with supplementation of plant growth regulators. Leaf, internode and node explants were inoculated in MS medium containing 0.25 – 4.0 mg/L TDZ and 2, 4-D. Significant callus (89.3± 3.8%) and shoot induction (71.8±3.1%) was observed in MS medium with 4 mg/L TDZ. Caulogenesis was achieved on the same medium used for callus induction. An average of 24.4±2.2 shoots having 4.01±0.2 cm length were regenerated in the culture. The highest rooting response (93.7±2.0%) was observed on the medium with 1.0 mg/l IBA with a mean number of 4.9±0.7 roots per shoot with mean length of 4.7±0.2 cm. Well developed plantlets were transferred to shade net with 100% successful acclimatization. This system may prove to be beneficial for ex-situ conservation as well as for amenability of this plant for genetic manipulation. This is the first report of indirect organogenesis in R. cordifolia with significantly high plantlet regeneration frequency.