alexa AQUEOUS TWO PHASE EXTRACTION OF FUNGAL AMYLASE AND ITS


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Research Article

AQUEOUS TWO PHASE EXTRACTION OF FUNGAL AMYLASE AND ITS USE FOR DESIZING OF COTTON FABRICS

Sunita singh*
Amity Institute of Biotechnology, Amity University Noida Uttar Pradesh, India
Corresponding Author: Sunita singh, Mob: 919582506614, E-mail: [email protected] [email protected]
Received: 04 August 2014 Accepted: 25 August 2014
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Abstract

The aqueous two phase system has a great potential for use in the downstream processing of fermentation products.The partitioning of fungal amylase enzyme in different concentration of PEG(30%-60%) and different molecular weight of PEG(4000,6000,8000) with 20%Sodium Sulphate system were investigated to optimized the amylase recovery.Maximum amylase activity (50U/ml) was found in 50%PEG 6000 and 20% Sodium sulphate system.Partially purified alpha amylase on desizing of cotton cloth was evaluated with varying incubation temperature from 400C to 800C for 1 hour. The highest desizing activity was found at 700C.The optimum temperature of partially purified enzyme was observed at 700C and maximum activity was found at pH7.Enzyme activity was increased with increase in substrate concentration up to 3%.

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