BIODEGRADATION OF PETROLEUM HYDROCARBONS POLLUTANTS IN SOIL USING MICROBIAL CONSORTIUM
|Deepika Paramanik and G.Rajalakshmi
PG and Research Department of Biotechnology, Hindusthan College of Arts and Science, Behind Nava India, Coimbatore â641028, Tamil Nadu, INDIA.
|Received: 21 June 2013 Accepted: 29 June 2013|
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Soil samples were collected from oil contaminated sites at Coimbatore. MTCC cultures, Micrococcus sp., Bacillus sp., Pseudomonas sp, which are able to utilize the oil in soil as carbon source, were added to oil contaminated soil samples. The growth profiles were determined by monitoring the optical density, dry weights and pH of the culture utilizing lubricating oil as sole carbon and energy source. They were incubated at 30ÃÂC for one week. Optimization was done based on the pH, temperature and nutrient contents. The microbial consortium showed optimum growth in day 21. The optical density varied from 0.543- 7.279. The gravimetric analysis showed the microbial consortium was capable of utilizing 80-90% the oil samples, under laboratory conditions at 30ÃÂC and 170rpm with Bushnell-Hass medium. Correlation analysis indicated positive correlation between optical density and pH (correlation coefficient = 0.735). After the extraction, the quantity of hydrocarbon content present in the soil sample was determined using GC- MS. Gas chromatographic analysis showed the degradation for petrol, diesel, and kerosene are 48%, 78% and 57% respectively. The statistical analysis was done with Students T-Test with p<0.05.These can be applied to oil contaminated area for cleanup process.