Chemical Derivatization UV Spectrophotometric Method for Detection of P-Aminophenol and Energy of Activation Approach to Set Degradation Protocol for Forced Degradation Studies
A spectrophotometric method has been developed for the determination of paracetamol in a dosage form and in Pharmaceutical preparation. The method is based on the reaction of p-aminophenol group of the drug with ninhydrin in N, N. dimethylformamide (DMF) medium producing a coloured complex which absorbs maximally at 547 nm. Beers law is obeyed in the concentration range 1-3μg/ml and with set parameter the method is validated, LOD and LOQ were found to be 0.254, 0.770 and 0.2849, 0.863 by standard deviation of blank and calibration curve method respectively. As it is having least sum square error that is 1.448667 × 10 -5. Method is found to be specific for the para amino phenol, data supported by ANOVA test with at P ≤ 0.05. For accuracy and precision we are 99Ã¢ÂÂ sure that the results lie between 100.32-99.26Ã¢ÂÂ and 100.19 to 100.07Ã¢ÂÂ respectively. From the principle of energy of activation the force degradation of Paracetamol in tablet dosage form is carried out at 100Ã¡ÂµÂc for 3 days, degradation of Paracetamol was found to be 0.7Ã¢ÂÂ Hence the method is developed and validated for the detection of p-amino phenol, a degradation product of paracetamol and by using concept of energy of activation p-amino phenol from paracetamol drug product is estimated the results were validated statistically.