COMPARISON OF ELISA AND RT-PCR FOR THE DETECTION OF PEANUT BUD NECROSIS VIRUS IN ONION (ALLIUM CEPA.L)
Peanut bud necrosis virus (PBNV) is an important re-emerging viral pathogen in onion (Allium cepa L.) in India. The virus transmitted by thrips vectors; it belongs to the genus Tospovirus and family Bunyaviridae. The onion crop infected by PBNV and it is a major problem in Southern India. This paper presents the comparison of DAC-ELISA and RT-PCR in the detection of PBNV infected onion samples from South India. The PBNV suspected onion samples (n=145) were collected in the major growing areas of Andhra Pradesh, Tamil Nadu and Karnataka states from South India. Among these collected onion samples, Seventy five samples (51.72%) were confirmed as PBNV infected by DAC-ELISA using the PBNV specific antiserum and in RT-PCR method one hundred twenty four samples (85.51%) were amplified (~800bp) by using the PBNV-CP gene specific primers. In comparison studies the RT-PCR method has added the advantage that it is more sensitive than the DAC-ELISA in the detection of PBNV in onion.