Detection and quantification of Uromyces setariae-italicae in foxtail millet by a real-time PCR assayNan Wang, Zhiping Dong, Hui Bai, Li Dong, Yongfang Wang, Jianzhang Quan, Lei Liu, Zhiyong Li*
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, P.R. China
- *Corresponding Author:
- Zhiyong Li
Millet Institute, Hebei Academy of Agricultural and Forestry Sciences, National Foxtail Millet Improvement Center, Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang, China, P.R. China
E-mail: [email protected]
Received date: 08/21/2015 Accepted date: 12/10/2015 Published date: 12/17/2015
Rust is a plant disease that has posed a serious problem to foxtail millet cultivation in Northern China since 1980. In this study, we developed a real-time fluorescence, quantitative PCR assay to accurately quantify genomic DNA of U. setariae-italicae based on the ITS region of rDNA gene. The resulting assay was highly species-specific, and was insensitive to DNA of other fungal isolates. The limit of quantification of the assay was 10 fg/ μl, and it was able to detect the rust fungus in foxtail millet leaves 6 h post inoculation. This work is mainly about the development of a molecular tool for the detection of U. setariae-italicae and will be helpful in diagnosis of rust infections in foxtail millet.