alexa Development and Validation of HPTLC Method for Simultan


Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Development and Validation of HPTLC Method for Simultaneous Estimation of Alogliptin Benzoate and Pioglitazone Hydrochloride in Bulk Drugs and Combined Dosage Forms

 
To read the full article Peer-reviewed Article PDF image

Abstract

A new, simple, precise, accurate and selective high performance thin-layer chromatographic (HPTLC) method has been developed and validated for the simultaneous estimation of Alogliptin benzoate (ALG) and Pioglitazone hydrochloride (PIO) in bulk and tablet dosage form. Chromatographic separation was carried out on Merck HPTLC aluminium sheets of silica gel 60F254 using Acetonitrile: 1% ammonium acetate in Methanol (4.5:5.5 v/v) as mobile phase followed by densitometry analysis at 254 nm. The reliability of the method was assessed by evaluation of linearity (500-3000 ng/spot for Alogliptin benzoate as well as for Pioglitazone). The accuracy of method was assessed by recovery studies and was found to be within range of 98-102% for both Alogliptin benzoate and Pioglitazone. The developed method was validated with respect to linearity, accuracy (recovery), and precision. The results were validated statistically as per ICH Q2 R1 guidelines and were found to be satisfactory. Due to non-availability of product, the simulation was done by using Piosys® tablets (Pioglitazone 30mg) and API of Alogliptin benzoate. The proposed method was successfully applied for the determination of Alogliptin benzoate and Pioglitazone in the mixture.

Keywords

Recommended Journals

Share This Page

Additional Info

Loading
Loading Please wait..
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords