alexa Development and Validation of Liquid Chromatography (RP-HPLC) Methodology for Estimation of Efonidipine HCl Ethanolate (EFD)
ISSN : 2153-2435

Pharmaceutica Analytica Acta
Open Access

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Research Article

Development and Validation of Liquid Chromatography (RP-HPLC) Methodology for Estimation of Efonidipine HCl Ethanolate (EFD)

Kumar A1,2* , Shoni SK1, Dahiya M1, Kumar R1, Yadav AK1, Kumar V2 and Chaudhary H2

1Indian Pharmacopoeia Commission (IPC), Ministry of Health and Family Welfare, Govt. of India, India

2PDM Colleges of Pharmacy, PDM University, Haryana, India

*Corresponding Author:
Akshay Kumar
PDM University, PDM College of Pharmacy
Sarai Aurangabad, Bahadurgarh, India
Tel: + 918901451603
E-mail: [email protected]

Received date: May 02, 2017; Accepted date: May 17, 2017; Published date: May 25, 2017

Citation: Kumar A, Shoni SK, Dahiya M, Kumar R, Yadav AK, et al. (2017) Development and Validation of Liquid Chromatography (RP-HPLC) Methodology for Estimation of Efonidipine HCl Ethanolate (EFD). Pharm Anal Acta 8:547. doi: 10.4172/2153-2435.1000547

Copyright: © 2017 Kumar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A Reversed Phase High Performance Liquid Chromatographic (RP-HPLC) method using symmetry C18, 5.0 mm column was developed for the determination of Efonidipine Hydrochloride Ethanolate (EFD). The mobile phase acetonitrile and water ratio was selected 85: 15 via flow rate were 0.8 mL/min and elution was monitored at 254 nm. Response was a linear function of concentration over the range 20-140 μg/ml (R2=0.9994) and the limits of detection was 681.83 ng/ml. The limit of quantification was 2.06 μg/ml. The coefficient of variation for intra-assay and inter-assay precision was less than or equal to 1.5% and the accuracy was 104.0-105.0% and method was validated accordance with International Conference on Harmonization (ICH) guidelines to check content uniformity. In this lieu, a simple and rapid with good accuracy precision validated method is developed which is applicable in quality-estimation, in-future.

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