Development and Validation of Liquid Chromatography (RP-HPLC) Methodology for Estimation of Efonidipine HCl Ethanolate (EFD)Kumar A1,2* , Shoni SK1, Dahiya M1, Kumar R1, Yadav AK1, Kumar V2 and Chaudhary H2
- *Corresponding Author:
- Akshay Kumar
PDM University, PDM College of Pharmacy
Sarai Aurangabad, Bahadurgarh, India
Tel: + 918901451603
E-mail: [email protected]
Received date: May 02, 2017; Accepted date: May 17, 2017; Published date: May 25, 2017
Citation: Kumar A, Shoni SK, Dahiya M, Kumar R, Yadav AK, et al. (2017) Development and Validation of Liquid Chromatography (RP-HPLC) Methodology for Estimation of Efonidipine HCl Ethanolate (EFD). Pharm Anal Acta 8:547. doi: 10.4172/2153-2435.1000547
Copyright: © 2017 Kumar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A Reversed Phase High Performance Liquid Chromatographic (RP-HPLC) method using symmetry C18, 5.0 mm column was developed for the determination of Efonidipine Hydrochloride Ethanolate (EFD). The mobile phase acetonitrile and water ratio was selected 85: 15 via flow rate were 0.8 mL/min and elution was monitored at 254 nm. Response was a linear function of concentration over the range 20-140 μg/ml (R2=0.9994) and the limits of detection was 681.83 ng/ml. The limit of quantification was 2.06 μg/ml. The coefficient of variation for intra-assay and inter-assay precision was less than or equal to 1.5% and the accuracy was 104.0-105.0% and method was validated accordance with International Conference on Harmonization (ICH) guidelines to check content uniformity. In this lieu, a simple and rapid with good accuracy precision validated method is developed which is applicable in quality-estimation, in-future.