DEVELOPMENT OF PROTOCOL FOR MASS MULTIPLICATION OF TWO ELITE VARIETIES OF SUGARCANE THROUGH MICROPROPAGATION
|Tarafdar S, Meena R*, Dhurandhar K, Pandey V, Vipani C and Thakur S
Aditya Biotech Lab & Research Pvt. Ltd., R&D Unit - Aditya Biotech Agricon Research & Development (ABARD) Centre, Raipur, Chhattisgarh
|Corresponding Author: Meena R, E-mail: [email protected]|
|Received: 10 January 2014 Accepted: 16 February 2014|
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Sugarcane (Saccharum officinarum L.) also called "noble canes" by Dutch scientists, belongs to family Poaceae and genus Saccharum. Sugar cane is the main source of sugar in all tropical and subtropical countries of the world with an annual production of 40 - 41 million tons. Sugar i.e. sucrose is necessary part of daily diet and a major portion of total sugar produced is used in alcoholic beverages, soft drinks, ice-creams, chocolates, canning industries and its by-products i.e. molasses and bagasse are also utilized as raw material for manure, fiber board and paper making units and even as fuel in sugar mills. Sugar cane is a tall, perennial grasses which is propagated vegetatively by stem cuttings called ‘setts’ having healthy buds. The conventionally propagated sets thus use a substantial number of canes and yet do not ensure uniformity, pest free or disease free status of planting material. The in vitro regeneration methods instead ensure large number of uniform, disease free and vigorous planting material in relatively lesser time. I this study we were emphases on the shoot tips containing auxiliary meristem were inoculated in MS medium with a fixed concentration of BAP (1mg/lit) for shoot tip initiation and establishment. Small shoots started appearing with in 7-10 days in all cultures bottles. Maximum shoot lengths were obtained in SM-II media i.e. MS supplemented with 0.25mg/lit BAP. After 30 days of incubation the average shoot length recorded in SM-II medium was found to be 1.63cm in Co-86032 and 1.65cm in C0-94012. Similarly the regenerated shoots were used for root induction in root forming media. The response of root formation was different in all media, as highest root formation average was observed in RM-III (3 mg/lit NAA & 3 mg/lit IBA) followed by RM-II media. Average number of plants showing roots was 9.6 in Co-86032 whereas it was recorded low in Co-94012 i.e. 7.8 in RM-III.