Effect of Different Growth Regulators on In-vitro Regeneration of Rhizome and Leaf Explants of Acorus calamus L.
The genus Acorus is a perennial aquatic plant used as a medicinal and aromatic plant. Acorus calamus were cultured for in-vitro investigation of growth rate, shoot formation and root formation on the media containing different kinds and concentrations of plant growth regulators. In the first experiment, different concentrations of sucrose (2, 4, 6, 8, and 10% w/v) were examined to find the most effective concentration in induction of rhizome using the dual phase MS medium. The tested concentration of sucrose was able to induce microrhizomes in the works. The 6 % sucrose medium had the largest size (length 3.9 cm, diameter 0.47cm) and maximum fresh weight (0.72 g). The explants were cultured in various concentrations of cytokines like BAP and Kn and auxins like IAA, IBA, NAA, 2, 4-D individually as well as in diverse combinations. Highest numbers of shoots were observed with 3.0 mg/L NAA. Half strength MS medium having 1.0 mg/L IBA was found better with 50 percent of root formation after 15 days of vaccination with a upper limit number of roots (5) in the platelets were transferred to the greenhouse and survival rate was 95% after acclimatization.