Effect of Short Term Exposure of Butylparaben on Total Uterine Tissue Protein in Ovariectomised C3H Albino Mice
Butylparaben is a widely used preservative in many pharmaceuticals and personal care products (PPCP) for its efficiency in preventing microbial growth in the products where it is added. However the scientist has been drawn attention to the wide use of this compound as it is found in breast cancer tissue. Studies in different experimental models including rodents have shown that it shows uterotrophic response thereby showing estrogenic activity. Butylparaben is found to show a weak estrogenic response in many in vitro systems. However the effect of butylparaben on uterus in mice is found to show sensitivity differences. In this experiment, the effect of butylparaben on the total uterine protein was studied by lowry method. Three doses of butylparaben 10 mg/Kg body weight/day, 50 mg/Kg body weight/day, 100 mg/Kg body weight/day was administered for 7 consecutive days through subcutaneous route of administration. In the experiment olive oil was used as vehicle control and 17β estradiol was used as positive control. After the short-term exposure of 7 days, butylparaben was found to increase in total uterine tissue protein in dose 10 mg/Kg body weight/day, 50 mg/Kg body weight/day and 100 mg/Kg body weight/day was found to increase in total uterine tissue protein. 17β estradiol, a potent natural estrogen caused a significant increase in total uterine tissue protein compared to vehicle control.