Evaluation of Methanolic Extract of Cleome chelidonii for Hepatoprotective Activity against Paracetamol and Ethanol Induced Hepatotoxicity in Rats
Objective: Traditionally Cleome chelidonii (Cleomaceae) used by tribal’s for the treatment of many liver disorders. The aim of this work was to evaluate the in vitro antioxidant and hepatoprotective activity of methanolic extract of Cleome chelidonii against paracetamol and ethanol induced liver toxicity in rats. Methods: The in vitro antioxidant activity was evaluated by estimating the lipid peroxidation, superoxide radical, hydroxyl radical and DPPH radical scavenging activities. Hepatotoxicity was induced in rats by oral administration of paracetamol (2 g/kg) and ethanol (2ml/100g) body weight. Blood samples were collected from retro orbital puncture from each rat and the Serum alkaline phosphatase (ALP), Serum glutamate oxaloacetate transaminase (SGOT), Serum glutamate pyruvate transaminase (SGPT) and Total Bilirubin (TBIL) levels were estimated. Results: The methanolic extract of cleome chelidonii and ascorbic acid at different concentrations scavenged the superoxide radicals, lipid peroxidation, hydroxyl radicals and DPPH radical in a dose dependent manner. The curative treatment with the methanolic extract of cleome chelidonii at 100, 200 and 400 mg/kg body weight and standard silymarin (100 mg/kg) was found to be prevent the rise in Serum ALP, SGPT, SGOT and TBIL levels due to paracetamol and ethanol treatment. The extract showed better effect in inhibiting the free radicals in in vitro studies which might be partly responsible for its beneficial action against paracetamol and ethanol induced liver damage. Conclusion: The free radical scavenging activity of the extract might be responsible for its protective activity against paracetamol and ethanol induced hepatotoxicity in rats.