Evaluation of Piperine Content from Roots of Piper Longum Linn., Originated from Different Sources with Comparison of Zonal Variation in Odisha, India
Plant phytochemicals are found to be responsible for various pharmacological effects on human health care system. Piperine, the principle bio-molecular active compound of Piper longum, elicits diverse pharmacological activities like analgesic, anti-pyretic bioavailability enhancer, antioxidant, immune-stimulant, hepatoprotective and many more. It is generally extracted or traditionally obtained from fruits (pippali) and roots (pippalimula) of wild Piper longum, a RET medicinal plant of the family Piperaceae. In this study, piperine content was assessed through Spectrophotometric and HPLC method from roots of Piper longum, collected from various agro-climatic zones of Odisha. Precisely the roots taken for experiment were originated from three types of cuttings used for propagation viz. nodal, Petiolar and apical. Through Spectrophotometric method of analysis piperine content in P. longum roots extracted through Methanol ranges in between 0.0176-1.424% dry wt. and ethanol extracted samples in between 0.0094-0.267% dry wt. Similarly in case of HPLC analysis, piperine content, extracted through Methanol ranges in between 0.104-0.74% dry wt. and ethanol extracted samples in between 0.042-0.363% dry wt. From the experiment Piperine content was appraised to be highest in roots of plants grown through nodal cuttings followed by Petiolar and apical leaf cuttings. Similarly amongst the selected agro-climatic zones, Keonjhar region showed highest yield of piperine in root samples followed by G. Udayagiri and finally by Khurda. Moreover among the selected solvent systems, methanol showed promising result in extraction process of piperine than ethanol.