EXPERIMENTAL VACCINATION OF SHEEP AGAINST HARD TICKS (IXODIDAE) USING WHOLE CRUDE LARVAE EXTRACT OF HYALOMMA ANATOLICUM ANATOLICUM IN SULAIMANI GOVERNORATE-IRAQ
Dr. Bahzad* and H. Salih Mustafa
Department of Animal Production-Faculty of Agricultural Sciences-Sulamani University-Sulaimani governorate-Iraq
|Corresponding Author: Dr. Bahzad, E-mail: [email protected]|
|Received: 08 October 2012 Accepted: 13 October 2012|
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The present study was carried out at October to December months 2011, unfed larval of Hyalomma anatolicum anatolicum were used for preparation of larval antigen for sheep (local breed) were inoculated subcutaneously (s/c) with 0.1 ml (8.2 mg/ml) in five successive doses of weekly interval. In each week end, five ml of blood was drawn directly from jugular vein for evaluation of gamma immunoglobulin (IgG) response. In this study there was that the highly correlation differences (r² = 0.976, p < 0.01) between all inoculation (immunization) weekly intervals with control sheep. Immunoglobulin-G level concentration in fifth week 2761.5 mg/dl was highly significant differences when comparison with others level values 1448.1 mg/dl , 1847.8 mg/dl in and 2097.7 mg/dl of other weeks 1st, 2nd and 3rd week’s immunization respectively, while showed no significant differences in immunoglobulin- G concentration with fourth inoculation sheep 2703.3 mg/dl. In this study observes that the increasment of immunization sheep percentage between each weekly immunization when compared with the control group, and revealed a highly resistance when exposed to challenge against of larval infestation in site of ear-pinnae.