GENOTOXICITY ASSESSMENT OF WATER EXTRACT OF OCIMUM GRATISSIMUM L. USING THE ALLIUM CEPA ASSAY
|Dimpy Das Borooah*
Centre for studies in Biotechnology, Dibrugarh University-786 004, Assam
|Corresponding Author: Dimpy Das Borooah,Centre for studies in Biotechnology, Dibrugarh University-786 004, Assam, E-mail:[email protected]|
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Aqueous leaf extract of Ocimum gratissimum was used to investigate its cytotoxic and genotoxic effects on root meristematic cells of Allium cepa. 1, 2.5, 5, 10 and 20% concentrations of leaf extract were used in the present study. It was found that leaf extract of O. gratissimum exhibits mitodepressive activity. Mitodepressive acitity was found maximum at high concentrations. Of all the concentrations used in the study, 5% concentration of leaf extract induced maximum genotoxicity leading to formation of sticky chromosome, c-metaphase, metaphasic and anaphasic disorders while 20% concentration showed binucleate cells.