IDENTIFICATION OF ANAEROBIC RUMEN FUNGI USING MOLECULAR METHODOLOGIES BASED ON RIBOSOMAL ITS1
|1Abbas Moazami Goudarzi, 2*Mohammad Chamani, 3Naser Maheri-Sis, 4Mehdi Amin Afshar and 5Ramin Salamatdoost-Nobar
|Corresponding Author: Mohammad Chamani, E-mail: [email protected]|
|Received: 05 May 2014 Accepted: 17 June 2014|
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Ruminal anaerobic fungi are important contributors to the utilization of poor-quality, high fiber pastures and crop residues by ruminants. Nucleic acid-based techniques which can be used to characterize complex microbial communities without incubation are now being employed regularly in ruminant nutrition studies. The foundation of the molecular ecology techniques is ITS1 rDNA sequence analysis which has provided a phylogenetically based classification scheme for identification of rumen fungi members. In this research, we try to determine the genetic diversity of the gastrointestinal tract anaerobic fungi in buffalos of Iran. After the sampling of the rumen contents, the genomic DNA was extracted from 10 fungal samples and then the electrophoresis was done to confirm the operations accuracy and after that, the PRC cultivation of ITSI region from the rRNA genes. The primers were GM1 and GM2. The phylogenetic tree was drawn using the Neighbor-joining method and the MEGA software. Sequence analysis of ITS1 spacer seems a promising tool for comparing a variety of rumen fungal isolates.