Immunological response of Lampito mauritii (Kinberg) coelomic fluid determined by hemolytic activity and sperm cytolysis
| J. Marlin Cynthia1*, K. Uma2, R. Gouthami2
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Earthworms have proved to be excellent inexpensive animals for studying regeneration, aspects of neurophysiology, endocrinology and excretion. The body cavity of annelids contains coelomic fluid (CF), in which coelomocytes, the worm’s leukocytes, are suspended. The component of CF of Earthworm defenses them in identifying foreign substance and develops potency to fight against them. The present study is the focused on the lytic effect of CF of Lampito mauritii on the vertebrate blood and sperm would pave the way to trace the immune phylogenetic significance. The Coelomic Fluid (CF) was collected and Hemolytic activity was determined and cytolysis of sperms was observed. The results of the present study reveal that the coelomic fluid is 100% haemolytic to the RBCs of fish, frog, calotes, goat and human. The coelomic fluid serially diluted showed gradual reduction in the hemolytic activity. It is evident that cytolysis and Haemolysis are the properties of coelomic fluid proteins which act as the humoral defense proteins to fight against invading antigenic cells. The coelomic fluid is a fluid system which allows these proteins too efficiently interact with the foreign cells, either bacteria or any vertebrate blood cells and even sperms.