IN VITRO PROPAGATION OFPOLIANTHES TUBEROSA L. CULTIVARS (CALCUTTA SINGLE)
|Jitendriya Panigrahi*, Mohammad Shahid L. Saiyad
Department of Biotechnology, Shri A. N. Patel P.G. Institute, Anand, GUJARAT-388001
|Corresponding Author: Jitendriya Panigrahi, Department of Biotechnology, Shri A. N. Patel P.G. Institute, Anand, GUJARAT-388001,Mob: +91 98981 50682, Email: [email protected]|
|Received: 16 May 2013 Accepted: 24 May 2013|
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The tuberosa occupies a very selective and special position among the ornamental bulbous plants for its attractive beauty, elegance and sweet pleasant fragrance. It has a great economic potential for cut-flower trade and essential oil industry. Due to its great demand, it has been cultivated in most of the tropical and sub-tropical countries. The main objective of the present investigation was to develop a dependable protocol for large scale clonal multiplication of Polianthes tuberosa variety like Calcutta Single under in-vitro conditions by using different combination and concentrations of growth hormones in MS medium. Among the various hormones used for multiple shoot formation the best response was produced with MS medium containing 0.2 mg/l of BAP and Kinetin with an average of 3.5±0.2 shoots of Calcutta Single. Shoots excised from cultures of proliferating shoots were rooted in half-strength MS medium having 0.5 mg/l IAA and BAP. In vitro rooted shoots from plantlets grew luxuriantly under field conditions and came to flowering after 1 month of transplantation.