IN VITRO RHIZOGENESIS FROM LEAF AND STEM CALLUS OF HELIOTROPIUM INDICUM, L.-MEDICINAL HERB
|Asha N Bagadekar and M Jayaraj
Department of Botany, Karnatak University, Dharwad, Karnataka, India.580003
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Heliotropium indicum, L. is one of the most common medicinal plants used by diverse cultures and tribal groups. In vitro callogenesis and rhizogenesis is achieved from different explants in stem and leaf of Heliotropium indicum, L Explants were incubated on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of auxins like 2,4-D, NAA and IBA at 1.0mg/L-3mg/L and cytokinins like Kn and BAP at 2mg/L. In the present study on Heliotropium indicum, L indicated that there is a strong ability for in vitro rhizogenesis than caulogenesis.This ethnomedicinal herb is known to have unique medicinal properties. The roots are bitter, astringent, aphrodisiac, expectorant, febrifuge and ophthalmic and are useful in cough, bronchitis, fever, ringworm. Ethnolic crude extracts of roots of Heliotropium indicum L., showed the presence of alkaloids, steroids, and carbohydrates. This provides a basis for germplasm conservation and for further investigations of bioactive constituents of roots of this plant.