IN VITRO SEED GERMINATION, CALLUS INDUCTION AND GERMPLASM CONSERVATION IN PALO DE BALSA [OCHROMA PYRAMIDALE (CAV. EX LAM.) URBAN.]
|Vilma E. Morante-Alarcón, Consuelo Rojas-Idrogo and Guillermo E. Delgado-Paredes*
Facultad de Ciencias Biológicas, Universidad Nacional Pedro Ruiz Gallo, Ciudad Universitaria, Juan XXIII 391, Lambayeque, Perú
|Corresponding Author: Guillermo E. Delgado-Paredes, Facultad de Ciencias Biológicas, Universidad Nacional Pedro Ruiz Gallo, Ciudad Universitaria, Juan XXIII 391, Lambayeque, Perú, E-mail: [email protected]; [email protected]; [email protected]|
|Received: 04 September 2014 Accepted: 11 October 2014|
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Palo de balsa [Ochroma pyramidale (Cav. ex. Lam. Urban.] is a tropical timber tree native of the Americas from Mexico and the West Indies to southern South America. It is an important species in both economic and ecological terms and their wood is precious and is used internationally for model making and locally for rafts, and the kapok for stuffing cushions.The highest percentage of seed germination (75%) was obtained in the treatment 1.0 mg/L GA3– 2% sucrose. Callus induction and germplasm conservation in explants derived from cultured seedlings were investigated in response to different plant growth regulators. Friable callus induction from hypocotyls and roots was performed on 2.0 mg/L NAA, in the case of auxins, and hypocotyls 2.0 mg/L BAP, and 1.0 and 2.0 mg/L 2iP, in the case of cytokinins. The best treatment on germplasm conservation was MS full-strenght supplemented with 0.5 mg/L ABA. In the re-growth process, the best treatment was MS medium with 0.02 mg/L IAA and 0.02 mg/L GA3.