alexa In-Vitro Anti-Inflammatory and Anti-Arthritic Activity of Hydroalcoholic Extract of Pongamia Pinnata (L.) Pierre Seed


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Research Article

In-Vitro Anti-Inflammatory and Anti-Arthritic Activity of Hydroalcoholic Extract of Pongamia Pinnata (L.) Pierre Seed

 
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Abstract

Objective: To evaluate Anti-inflammatory and Anti-arthritic Activity of seed extract of Pongamia pinnata (L.) Pierre by in vitro model. Materials and methods: P. pinnata (L.) Pierre (Family: Leguminoseae) is a medicinal plant which is indicated for the treatment of arthritis in folklore medicine. The present study was aimed at the investigation of anti-arthritic and anti-inflammatory activity in hydroalcoholic extract of P. pinnata. The anti-arthritic and anti-inflammatory activity of P. pinnata hydroalcoholic extract was done by Inhibition of protein denaturation and Human red blood cell membrane stabilization (HRBC) in vitro methods. The hydroalcoholic extract of P. pinnata was subjected to in vitro Inhibition of protein denaturation in various concentrations i.e. 10, 50, 100, 200, 400, 800, 1000 and 2000μg/ml. HRBC method was also used for the estimation of anti-inflammatory activity from in various concentrations 100, 200, 400, 800 and 1600 μg/ml. Results: P. pinnata hydroalcoholic extract exhibited a concentration dependent inhibition of protein (albumin) denaturation. The stabilization of HRBC membrane showed a concentration dependent anti-inflammatory activity, and the protection percent increased with increase in the concentration of the P. pinnata hydroalcoholic extract. Conclusion: The present study is support to the isolation and use of phytoconstituents from seed of P. pinnata in treatment of inflammation and arthritis.

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