INVITRO MICROPROPAGATION OF EULOPHIA NUDA LIND AN ENDANGERED TERRESTRIAL ORCHID THROUGH PLBÂ’ (PROTOCORM LIKE BODIES)
|Mrs.Ruchi Kurapa Shroti and Ravi Upadhyay
Government P. G. College Pipariya, M.P
|Received: 21 December 2013 Accepted: 30 December 2013|
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An efficient and reproducible protocol developed for micropropagation of Eulophia nuda L. an endangered terrestrial orchid; using 4mm section of axillary bud segment has been developed. Axillary bud section were cultured on Murashige and Skoog (MS) medium supplemented with different concentration of harmones (BAP), 5,1.0,2.0,mg/L, [α-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), 0.5, 1.0, 2.0 and 5.0 μM] and Casein hydrolysetes (CH: 5, 10 and 15%).,2,4-D,.2,.5,1.0, 2.0.The explants developed protocorm like bodies (PLBs) within 6–8 weeks on the growth medium. MS+2 mg/l 2,4-D supplemented medium was found best for the induction of PLBs. Upon subculture on basal MS medium, the PLBs differentiated plantlets within 6–8 weeks with in vitro tuber and roots. This simple protocol will be useful for large-scale propagation of Eulophia nuda lind.