alexa Isolation, Identification and Characterization of Cellulose-Degradation Bacteria from Fresh Cow Dung and Fermentation Biogas Slurry
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
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Research Article

Isolation, Identification and Characterization of Cellulose-Degradation Bacteria from Fresh Cow Dung and Fermentation Biogas Slurry

Zhou Hong-li1, Yang Xiao2, Xiong Dong-mei3, Zeng Lu3, Tian Kai-zhong3, Xiong Xing-yao3,4, Liu Yun2, Su Xiao-jun1,3,5*

1College of Food Science and Technology, Hunan Agricultural University, Changsha, PR China

2Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, PR China

3Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha, PR China

4The Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences, Beijing, PR China

5Hunan Collaborative Innovation for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, PR China

*Corresponding Author:
Xiaojun Su
College of Food Science and Technology, Hunan Agricultural University, Changsha, PR China
E-mail: [email protected]

Received date: 26 June 2015; Accepted date: 01 August 2015; Published date: 07 August 2015

 

Abstract

Two new isolates designated strain MY6 and strains FY2 were isolated from the mixture of fresh cow dung and fermentation biogas slurry, which were found to be potential cellulase producers. On the basis of cellular morphology, physiological and chemotaxonomic characteristics and similarity of 16S rDNA gene sequences, the former was confirmed as Stenotrophomonas sp., while the latter was identified as Bacillus cereus sp. Key fermentation factors including culture time, initial pH and culture temperature for cellulase production were optimized using single factor experiments for the two strains. The endoglucanase (CMCase) activity of MY6 was increased by 200.74% and reached 137.36 U/ml under the optimized conditions (cultured for 48 h at pH7.0 and 40°C with shaking at 160 rmp); while the activity of FY2 was enhanced by 150.61% and achieved 177.58 U/ml under the optimized conditions (cultured for 48 h at pH7.0 and 45°C with shaking at 160 rmp). The effects of four native lignocellulosic feed stocks on CMCase activities were compared. The results showed that cellulase from strains MY6 and FY2 were similar in cellulose degradation and the activity was strongest for filter paper, then for degreased cotton, straw powder and sawdust as substrates. These findings indicated that high cellulose concentration used as carbon sources could promote the generation of CMCase.

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