LIGATION OF E2 GENE WITH REPLICASE BASED EUKARYOTIC pSinCMV VECTOR AND ITS TRANSFORMATION STUDY
|Nitin Sharma*,Purshotam Kaushik1, Anant Rai2
|Corresponding Author: Nitin Sharma, E-mail: [email protected] Phone:-09897857585|
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The E2 gene of classical swine fever virus (CSFV) is already cloned in pVAX1 vector. In the present study we have released the E2 gene insert from the pVAX1.CSFV.E2 using plasmid isolation, RE digation and legated with pSinCMV vector. Prepared the competent cell to transformation of Ligated product in E.coli DH5α. Make the L.B. Agar plate and the large number of colonies (approximately 25) were picked from the overnight grown transformants. The individual colonies were inoculated in fresh ampicillin (50 μg/ml) containing LB broth and allowed to grow for large scale production for further experiments. This can be used for expression and immunological studies.