alexa One Step RT- PCR for Detection of Respiratory Syncytial
ISSN: 2319-9865

Research & Reviews: Journal of Medical and Health Sciences
Open Access

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Research Article

One Step RT- PCR for Detection of Respiratory Syncytial Virus in Children with Lower Respiratory Tract Infection

Kiran Yadav*, VG Ramachandran, Shukla Das and Dheeraj Shah1

Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi-110095, India

1Department of Paediatrics, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi-110095, India

*Corresponding Author:
Kiran Yadav
University College of Medical Sciences &
Guru Teg Bahadur Hospital
Delhi-110095, India
Mobile: +91 9266882083

Received: 17 August 2013 Accepted: 14 September 2013

 

Abstract

To assess the diagnostic utility of Reverse Transcriptase Polymerase chain reaction (RT-PCR) in detecting the respiratory syncytial virus in lower respiratory tract infection (LRTI). Cross sectional diagnostic accuracy study in tertiary care hospital. Total 130 cases with LRTI between 2 months to 5 years of age were included in the study. Flocked nasal swabs samples were processed for detection of RSV virus by both rapid antigen detection method and RT-PCR. RSV was detected in 14 patients by rapid method and by RT-PCR in 16 patients. Out of the 14 positive cases by rapid antigen method, 13 subjects were also positive by RT-PCR but one was negative. There were 3 more subjects, positive only by RT-PCR. The sensitivity and specificity of RT-PCR was 92.85 percent and 97.41 percent respectively. RSV was demonstrated in much higher percentage of bronchiolitis, especially below 6 months of age compared to pneumonia. RT-PCR assay is a highly sensitive method for detection of RSV. If an appropriate turnaround time can be offered, molecular detection should be used for RSV diagnosis.

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