Pharmacokinetic Study of [Di (4-amino N-acetyl) phenoxy]methyl ketone as Compared to Paracetamol
In the present study, comparative pharmacokinetics study between [Di (4-amino N-acetyl) phenoxy] methyl ketone (DPMK) and paracetamol in rats were investigated. DPMK and paracetamol were orally given to experimental rats in dose 500 mg/kg/b.wt. At various time intervals, blood (1 h - 24 h) and urine (6 h - 84 h) sample was collected from each rat. The drug concentrations were measured in blood supernatants and urine samples by using validated UV-visible spectrophotometric method. Various pharmacokinetic parameters were calculated by non compartmental model for DPMK and paracetamol. DPMK reached a higher concentration Cmax =22.26 ± 1.85 μg/mL after oral administration in rat as compared to standard paracetamol Cmax = 5.18 ± 0.57 μg/mL. There was a significant increased in the AUC (0-∞) = 85.82 ± 8.67 μg h/mL and AURC (0-∞) = 338.86 ± 53.76 mg of DPMK as compared to the paracetamol AUC (0-∞) = 20.51 ± 1.79 μg h/mL and AURC (0-∞)= 42.97 ± 1.58 mg. No significant differences were found in elimination rate constant during the PK study of DPMK and paracetamol. The apparent volume distribution (Vss = 7.24 ± 1.51 lit) and total clearance (CL = 171 ± 0.24 lit/h) for DPMK were found to be less as compared to the paracetamol (Vss = 29.40 ± 4.17 lit, CL = 7.43 ± 0.89 lit/h). Furthermore, the relative bioavailability of DPMK was found to be in the range 4.18-7.92 for both blood and urine sample.