alexa Possible Anti-nociceptive Action of Either Vitamin B1 or B12 Against Writhing Response Induced in Mice by Glacial Acetic Acid: Comparative Study with Tramadol.


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Research Article

Possible Anti-nociceptive Action of Either Vitamin B1 or B12 Against Writhing Response Induced in Mice by Glacial Acetic Acid: Comparative Study with Tramadol.

 
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Abstract

Objective: to evaluate whether or not vitamin B1 or B12 possess anti-nociceptive effect against writhing response induced in mice by glacial acetic acid; to compare that effect of each vitamin with tramadol and to clarify the mechanism of the proposed anti-nociceptive effect centrally of only vitamin B1 utilized in this study by administering naloxone (as opioid antagonist). Methods: 42 mice (Mus musculus) of both sexes are divided into 4 groups as follows: Group I. Six animals were given intraperitoneal injection (IP) of normal saline, then after 30 minutes they were given 1 ml I.P. injection of glacial acetic acid (0.6%.). This group served as untreated control. Group II. Eighteen mice were allocated as follows: IIA. Six animals were injected Vitamin B1 I.P 30 minutes prior to IP injection of 1 ml of glacial acetic acid (0.6%). IIB. Six animals were injected Vitamin B12 I.P 30 minutes prior to IP injection of 1 ml of glacial acetic acid (0.6%). IIC. Six animals were injected tramadol (20 mg/Kg) IP 30 minutes prior to IP injection of 1 ml of glacial acetic acid (0.6%). Group III. Twelve animals were allocated as follows: IIIA. Six animals treated with IP injections of a combination of tramadol (20mg / Kg) and Vitamin B1 (100mg/kg) given to mice 30 minutes prior to 1 ml I.P. injection of glacial acetic acid (0.6%.). IIIB. Six animals treated with IP injections of a combination of tramadol (20mg / Kg) with Vitamin B12 (1mg/Kg) given to mice 30 minutes prior to 1 ml I.P. injection of glacial acetic acid (0.6%.). Group IV. Six animals were injected IP with naloxone (0.73mg/Kg) were given to mice followed by I.P. infection of vitamin B1 (1mg/Kg) and after 30 minutes they were given 1 ml I.P. injection of glacial acetic acid (0.6%.). The number of writhes (abdominal constriction and full extension of hind limb) of each mouse was recorded after 5minutes of glacial acetic acid for a period of 10 min. Results: there were significant reduction in number of writhes produced by either vitamin B1 or B12 compared to untreated group; also there was significant difference produced by the combination of tramadol and B1 compared to vitamin B1 alone, and there was no significant difference between the combination of naloxone and vitamin B1 and untreated group. Conclusion: both vitamin B1 and B12 possess an anti-nociceptive effect in this animal module. Vitamin B1 acts via opioid mechanism (as pain modulator) manifested by returning to base line of pain when using naloxone (opioid antagonist) and the synergistic effect with the use of vitamin B1 with tramadol (opioid agonist).

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