PRETREATMENT OF AGRICULTURAL WASTE WITH PLEUROTUS SP. FOR ETHANOL PRODUCTION
|Jasmine Koshy* and Padma Nambisan
Plant Biotechnology Laboratory, Department of Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India.
|Corresponding Author: Plant Biotechnology Laboratory, Department of Biotechnology, Cochin University of Science and
Technology, Cochin-682 022, Kerala, India. Tel: +91-484 2576267, Fax: +91-484 2577595.,[email protected]
Bioethanol is a liquid fuel obtained from fermentation of sugar/starch crops. Lignocellulosic biomass being less expensive is considered a future alternative for the food crops. One of the main challenges for the use of lignocellulosics is the development of an efficient pre-treatment process. Pretreatments are classified into three - physical, chemical, and biological pretreatment. Chemical process has not been proven suitable so far, due to high costs and production of undesired by-products. Biologically, hydrolysis can be enhanced by microbial or enzymatic pretreatment. Studies show that the edible mushrooms of Pleurotus sp. produce several extracellular enzymes which reduce the structural and chemical complexity of fibre. In the present study, P. ostreatus and P. eous were cultivated on paddy straw. Spent substrate left after mushroom cultivation was powdered and used for ethanol production. Saccharomyces sp. was used for fermentation studies. Untreated paddy straw was used as control. Production of ethanol from P. ostreatus substrate was 5.5 times more when compared to untreated paddy straw, while the spent substrate of P. eous gave 5 times increase in ethanol yield. Assays showed the presence of several extracellular enzymes in the spent substrate of both species, which together contributed to the increase in ethanol yield.