alexa Screening of Phytochemical and Antimicrobial Activities of Azima tetracantha Lam. Leaf Extracts


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Research Article

Screening of Phytochemical and Antimicrobial Activities of Azima tetracantha Lam. Leaf Extracts

 
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Abstract

The medicinal plant Azima tetracantha was analysed for screening of phytochemical and antimicrobial activity. The In-vitro antimicrobial activity was studied against human pathogens gram positive strains Bacillus subtillis, Bacillus cereus and Staphylococcus aureus, and gram negative strains Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi and three fungal strains: Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus using disc diffusion method. Phytochemical analysis of Azima tetracantha leaf extracts result reveals that the hexane extracts showed the presence of alkaloids, phenol, protein and flavonoids, chloroform extracts showed the presence of alkaloids, glycosides, phenol, protein, flavonoids and tannins, ethyl acetate and methanol extracts showed the presence of alkaloids, glycosides, saponins, steroids, phenol, flavonoids, tannins and terpenoids. The In-vitro antibacterial activity of Azima tetracantha leaf showed the maximum zone of inhibition in methanol and ethyl acetate extract against gram positive bacteria S. aureus at 1000μg concentration. In-vitro antifungal activity of Azima tetracantha leaf showed the maximum zone of inhibition in methanol and ethyl acetate extracts against fungi A. fumigatus at 1000μg concentration. The In-vitro antibacterial activity of Azima tetracantha leaf showed the maximum zone of inhibition in chloroform and hexane extracts against gram positive bacteria B. cereus at 1000μg concentration. In-vitro antifungal activity of Azima tetracantha leaf showed the maximum zone of inhibition was found in chloroform and hexane extracts against fungi A. fumigatus at 1000μg concentration. This result justifies the presence of secondary metabolites and their activity

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