alexa Sex Determination in Gibbons of Genus Nomascus using No
ISSN: 2167-6801

Journal of Primatology
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Research Article

Sex Determination in Gibbons of Genus Nomascus using Non-invasive Method

Petra Bolechová1,3*, Kateřina Ječmínková1,2, Michal Hradec1, Tomáš Kott2 and Jana Doležalová4

1Department of Animal Science and Ethology, Czech University of Life Sciences Prague, Kamýcká 129, 16521, Prague Suchdol, Czech Republic

2Department of Molecular Genetics, Institute of Animal Science, Přátelství 815, 10400, Prague Uhříněves, Czech Republic

3Zoo Liberec, Masarykova 1347/31, 46001, Liberec 1, Czech Republic

4Department of Physiology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tř 1/3,61242, Brno, Czech Republic

*Corresponding Author:
Petra Bolechová
Department of Animal Science and Ethology, Czech University of Life Sciences Prague
Kamýcká 129, 16521, Prague Suchdol, Czech Republic
Tel: +420 22438 3621
E-mail: [email protected]

Received date: April 19, 2016; Accepted date: June 27, 2016; Published date: June 30, 2016

Citation: Bolechova P, Jecmínková K, Hradec M, Kott T, Doležalová J (2016) Sex Determination in Gibbons of Genus Nomascus using Noninvasive Method. J Primatol 5:133. doi: 10.4172/2167-6801.1000133

Copyright: © 2016 Bolechova P et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Gibbons of the genus Nomascus have a strong sexual dimorphism. As they mature, both sexes develop sex specific pelage colour. In combination with physical similarities in genitalia with both sexes, there are problems with determining the sex of young individuals compared to other genus of gibbons. This is a pilot study applying a multiplex polymerase chain reactions based on a non-invasive method for sex determination of gibbons. The study was conducted on 22 faecal samples from gibbons of genus Nomascus. The animals were monitored by staff in order the samples were identified correctly and each sample was collected immediately after the defecation. Results confirmed the sex in all adult and juvenile animals with known sex and we determined 2 females and 5 males in juveniles with unknown sex. The results of direct examination completely corresponded with the PCR results. The PCR reaction with template DNA isolated from feacal material required BSA usage; however, we observed the occurrence of nonspecific fragments. This did not affect the reliability of our results and we confirmed usability of this method for this genus.

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