alexa Sputum processing prior to Mycobacterium tuberculosis detection by culture or nucleic acid amplification testing: a narrative review
E- ISSN: 2320 - 3528
P- ISSN: 2347 - 2286

Research & Reviews: Journal of Microbiology and Biotechnology
Open Access

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Review Article

Sputum processing prior to Mycobacterium tuberculosis detection by culture or nucleic acid amplification testing: a narrative review

Veronica Allen1*, Mark P Nicol1,2,3, Lemese Ah Tow1

1Department of Pathology, Division of Medical Microbiology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa

2Institute for Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa

3National Health Laboratory Service, Groote Schuur Hospital, Cape Town, South Africa

*Corresponding Author:
Veronica Allen
Department of Pathology, Division of Medical Microbiology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa
E-mail: [email protected]

Received date: 22/12/2015 Accepted date: 09/03/2016 Published date: 29/03/2016

 

Abstract

Sputum is a complex specimen consisting of a primary network comprised of linked mucin molecules and a secondary network composed of filamentous actin, cell debris and DNA. Other components of sputum are leukocytes, proteoglycans, inflammatory mediators and elastin fibres. Embedded within this matrix are bacteria which are targeted for clinical diagnosis. This is further complicated in tuberculosis as mycobacteria frequently clump within the specimen resulting in unequal distribution. Efficient release of bacteria from sputum specimens requires chemical and/or mechanical breakdown of both primary and secondary sputum networks. This review gives an overview of the composition of sputum and of various methods that have been used for digesting sputum prior to testing for tuberculosis.

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