STUDIES ON THE MALE ACCESSORY SEX GLANDS AND EFFECT OF JUVENILE AND ECDYSONE HORMONE ON THE AQUATIC BEETLE, CYBISTER TRIPUNCTATUS (OL.)
In Cybister tripunctatus male accessory sex glands (MASG) are paired, long, coiled and tubular structures extending into the abdominal cavity and open into the lateral ejaculatory duct of its own side. The wall of the MASG is composed of a layer of tall columnar epithelial cells and the lumen is filled with granular secretory material. The histochemical studies revealed the presence of abundant quantity of DNA in the nuclei, RNA in the nuclei and cytoplasm suggesting protein synthesis in the epithelial cells of MASG in adult male. The histochemical tests suggested that the secretory material accumulated in the cells and lumen of the MASG is a mixture of protein, carbohydrate and lipid. The biochemical analysis showed that the secretory material of MASG composed of large quantity of protein and little amount of carbohydrate and lipid. The SDS – PAGE separated in all 17 protein bands ranging from 18.5 to 205.000 in molecular weight. Tropical application of JH-III on the mature male caused significant increase while that of β - ecdysone showed reduction in the total protein concentration of MASG.