THE STUDY OF COMPLEX VERTEBRAL MALFORMATION GENETIC DEFECT IN A POPULATION OF SISTANI COWS
Behzad Hemati1, Jaber Saberi*1, Ali Reza Noshary1
|Corresponding Author: Jaber Saberi, E-mail: [email protected]|
|Received: 4 April-2014 Accepted: 5 May-2014|
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In the present study, complex vertebral malformation (CVM) genetic defect was studied in a population of Sistani cows using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCRRFLP) method. The purpose of this study was to investigate carriers of CVM defect in a population of Sistani cows. In this genetic defect, dead calves would be born earlier than normal with disorders such as shorter neck, badly shaped legs, curved spines, connected ribs and heart disorders in some cases. Blood samples were collected by Venojects containing EDTA and then transferred to Research Laboratory of Islamic Azad University, Karaj branch. Polymerase Chain Reaction was performed for amplification of 233bp DNA fragments in Exon 4 of chromosome 3 of SLC35A3 gene using specific primers. Enzymatic digestion was performed on polymerase chain reaction products from SLC35A3 gene to identify carriers of CVM using Eco T221 enzyme and the restricted products were loaded on 1.5% Agarose gel.We didn’t find any affected cow with the mutant allele of SLC35A3 for CVM defect comparing with positive evidence. It seems that Sistani cows don’t have mutant allels for this gene.