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Research Article Open Access
Nyctanthesarbor-tristis Linn. belonging to family Oleaceae is a well-known medicinal plant. The leaves extracts of the plant were tested for their in vitro antimicrobial activity by agar well plate method. The test organisms were Staphylococcus aureus and Pseudomonas aeruginosa. Staphyloccusepidermis, Escherischia coli. The zone of inhibition and Minimum Inhibitory Concentration (MIC) of the extracts were determined and compared with the standard drug of ofloxacin. The Aqueous extract was found to have antibacterial activity. This method is based on the diffusion of an antibiotic from a well through the solidified culture media of a petridish used for study. Growth of inoculated microorganism is inhibited entirely in a circular area "Zone", around the well containing a solution of the antibiotic and the plant extract.