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Research Article Open Access
Gibbons of the genus Nomascus have a strong sexual dimorphism. As they mature, both sexes develop sex specific pelage colour. In combination with physical similarities in genitalia with both sexes, there are problems with determining the sex of young individuals compared to other genus of gibbons. This is a pilot study applying a multiplex polymerase chain reactions based on a non-invasive method for sex determination of gibbons. The study was conducted on 22 faecal samples from gibbons of genus Nomascus. The animals were monitored by staff in order the samples were identified correctly and each sample was collected immediately after the defecation. Results confirmed the sex in all adult and juvenile animals with known sex and we determined 2 females and 5 males in juveniles with unknown sex. The results of direct examination completely corresponded with the PCR results. The PCR reaction with template DNA isolated from feacal material required BSA usage; however, we observed the occurrence of nonspecific fragments. This did not affect the reliability of our results and we confirmed usability of this method for this genus.
Primates, Nomascus, Sex-typing, Multiplex PCR, Faeces, Primates, Primates Life