"Procuring of amyloid deposits was performed as previously described. Shortly, 8 Âµm thick tissue sections of formaldehyde fixed and paraffin embedded tissue were cut and placed centrally onnon-charged glass slides, followed by deparaffinization, staining with Congo red, and dehydration using standard histological procedures. The Arcturus Autopix Laser Capture Microscope (Applied Biosystem) was used to isolate amyloid deposits appearing red due to the Congo red staining. In addition, tissue with no apparent amyloid deposits was likewise procured and used as control tissue. Tissuewas collected on CapSure Macro LCM Caps (Applied Biosystem), subsequently placed in sterile 0.5 ml Eppendorf Biopur Microcentrifuge Tubes (VWR) and stored at -80Â°C until further processed.
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Citation: Nielsen NS, Poulsen ET, Klintworth GK, Enghild JJ (2014) Insight into the
Protein Composition of Immunoglobulin Light Chain Deposits of Eyelid, Orbital and
Conjunctival Amyloidosis. J Proteomics Bioinform S8: 002"
Last date updated on July, 2014