Adopting a tissue-engineering approach to this problem, research has moved along two different paths. The holy grail of tissue engineering, the in vitro production of a complete autologous organ, has been pursued with some success. Vacantiâs group repopulated a decellularized rat lung with stem cells and demonstrated a limited gas transfer of this tissue engineered lung during in vitro tests. After orthotopic transplantation in rats, the tissue engineered lungs retained their gas exchange capability for 6 hours before becoming edematous.
engineering of a biohybrid lung assist device seems an intermediary goal that might allow for enhanced biocompatibility of oxygenators by fabricating a confluent endothelial cell layer on top of the gas exchange membrane
Material characteristics have hampered surface endothelializationin several instances. Bengtsson and Haegerstrand implanted endothelialized mechanical heart valves made from pyrolytic carbon in pigs in the pulmonary position. The endothelial cells were swept away by the blood flow within one hour. Herring et al. observed similar results for the endothelialization of vessel grafts fabricated from Polytetrafluoroethylene. The Biohybrid LungâCurrent Perspective: Christian G Cornelissen and Stefan Jockenhoevel
Last date updated on July, 2014