Radioautography

"Radioautography can be defined as the procedure used to visualize the details using radio labelled compounds.The techniques employ both the physical techniques using RI-labeled compounds and the histochemical techniques treating tissue sections by coating sections containing RI-labeled precursors with photographic emulsions and processing for exposure and development. Such techniques can demonstrate both the soluble compounds diffusible in the cells and tissues and the insoluble compounds bound to the macromolecules. In various kinds of cells of various organ in respective organ systems such as skeletal, muscular, circulatory, digestive, respiratory, urinary, reproductive, endocrine, nervous and sensory systems of the human and various animals, in order to observe the localizations of the incorporations and syntheses of various nutrients synthesizing macromolecules in the human or animal bodies such as DNA, RNA, proteins, glucides and lipids using RI-labeled compounds, to demonstrate the localizations of macromolecular synthesis by using such RI-labeled precursors as 3H-thymidine for DNA, 3H-uridine for RNA, 3H-leucine for protein, 3H-glucosamine or 35SO4 for glucides and 3H-glycerol for lipids are divided into macroscopic radioautography and microscopic radioautography. (Tetsuji Nagata- Light and Electron Microscopic Radioautographic Studies on the Cell Aging of the Neuro-Sensory System of Mice)."

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Last date updated on April, 2024