"It is well known that microbial cells must keep an internal balance of electrical charges in order to maintain a functional cell membrane. This is achieved by maintaining a near-neutral pH and a more negatively charged cytoplasm than the external solution. Since N is a major nutrient for fungi, the form in which it is taken up by fungal cells can shift the electrical charge in the cytoplasm. Since the net charge in the cytoplasm must remain negative, imbalance caused by the uptake of excess cations must be countered by a very active proton- pumping, which expels H+ into the external medium through likely a K+/H+ antiport mechanism.
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Last date updated on July, 2014